Phosphorescent Oxygen-Sensitive Probes [electronic resource] /
by Dmitri B. Papkovsky, Alexander V. Zhdanov, Andreas Fercher, Ruslan I. Dmitriev, James Hynes.
- VIII, 101 p. 39 illus., 17 illus. in color. online resource.
- SpringerBriefs in Biochemistry and Molecular Biology, 2211-9353 .
O2-sensitive probes based on phosphorescent metalloporphyrins -- O2 analysis on a fluorescence spectrometer or plate reader -- O2 Imaging in biological specimens.
Biological O2 sensing probes and measurement techniques were first introduced in the late 80s. In the last 3-5 years they have undergone major development that have made them available and affordable for a broad range of applications in various disciplines of the life and biomedical sciences. These new chemistries and technologies, which are significantly different from the majority of other fluorescence-based probes and detection techniques, have already demonstrated their high utility. This book will provide a systematic overview of the existing and emerging O2 sensing technologies in their different modifications, a practical guide to their rational selection and use, and examples of biological applications/case studies, including details on how to set up and conduct such experiments, troubleshoot and interpret the data.