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03193nam a22004935i 4500 |
003 - CONTROL NUMBER IDENTIFIER |
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005 - DATE AND TIME OF LATEST TRANSACTION |
control field |
20140310150240.0 |
007 - PHYSICAL DESCRIPTION FIXED FIELD--GENERAL INFORMATION |
fixed length control field |
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008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION |
fixed length control field |
100301s2010 gw | s |||| 0|eng d |
020 ## - INTERNATIONAL STANDARD BOOK NUMBER |
International Standard Book Number |
9783642046094 |
|
978-3-642-04609-4 |
050 #4 - LIBRARY OF CONGRESS CALL NUMBER |
Classification number |
QR180-189.5 |
082 04 - DEWEY DECIMAL CLASSIFICATION NUMBER |
Classification number |
616.079 |
Edition number |
23 |
264 #1 - |
-- |
Berlin, Heidelberg : |
-- |
Springer Berlin Heidelberg, |
-- |
2010. |
912 ## - |
-- |
ZDB-2-SBL |
100 1# - MAIN ENTRY--PERSONAL NAME |
Personal name |
Buchwalow, Igor B. |
Relator term |
author. |
245 10 - IMMEDIATE SOURCE OF ACQUISITION NOTE |
Title |
Immunohistochemistry: Basics and Methods |
Medium |
[electronic resource] / |
Statement of responsibility, etc |
by Igor B. Buchwalow, Werner Böcker. |
300 ## - PHYSICAL DESCRIPTION |
Extent |
X, 153p. 39 illus. |
Other physical details |
online resource. |
505 0# - FORMATTED CONTENTS NOTE |
Formatted contents note |
Antibodies for Immunohistochemistry -- Antibody Labeling and the Choice of the Label -- Probes Processing in Immunohistochemistry -- Working with Antibodies -- Background Staining, Autofluorescence and Blocking Steps -- Immunostaining Enhancement -- Multiple Multicolor Immunoenzyme Staining -- Multiple Immunofluorescence Staining -- Antigen Detection on Tissues Using Primary Antibody Raised in the Same Species -- Probes for Staining Specific Cellular Organelles -- The Use of Epitope Tags in Histochemistry -- Immunohistochemistry at the Ultrastructural Level -- Diagnostic Immunohistochemistry -- A Picture Is Worth a Thousand Words. |
520 ## - SUMMARY, ETC. |
Summary, etc |
While many excellent texts and monographs exist which cover various aspects of immunohistochemistry, the lack of a concise comprehensive guide to using the field’s methods was a major motivation for the authors to write this book. The authors carefully selected the methods and easy-to-adopt protocols discussed. In addition to estabished techniques, special attention was paid to current developments in immunohistochemistry such as antigen retrieval, signal amplification, the use of epitope tags, multiple immunolabeling, and diagnostic immunohistochemistry. All procedures examined have been tested by the authors; many are routinely used in daily practice in their institute. Each chapter starts with a short introduction to the respective method’s underlying principles before presenting step-by-step protocols and instructions, including precise recipes for all materials needed. Thus, the book is suitable for a broad audience, ranging from students and technical assistants to experienced researchers. |
650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM |
Topical term or geographic name as entry element |
Medicine. |
|
Topical term or geographic name as entry element |
Immunology. |
|
Topical term or geographic name as entry element |
Monoclonal antibodies. |
|
Topical term or geographic name as entry element |
Medical laboratories. |
|
Topical term or geographic name as entry element |
Cytology. |
|
Topical term or geographic name as entry element |
Cytology |
General subdivision |
Research_xMethodology. |
|
Topical term or geographic name as entry element |
Biomedicine. |
|
Topical term or geographic name as entry element |
Immunology. |
|
Topical term or geographic name as entry element |
Biological Techniques. |
|
Topical term or geographic name as entry element |
Cell Biology. |
|
Topical term or geographic name as entry element |
Laboratory Medicine. |
|
Topical term or geographic name as entry element |
Antibodies. |
700 1# - ADDED ENTRY--PERSONAL NAME |
Personal name |
Böcker, Werner. |
Relator term |
author. |
710 2# - ADDED ENTRY--CORPORATE NAME |
Corporate name or jurisdiction name as entry element |
SpringerLink (Online service) |
773 0# - HOST ITEM ENTRY |
Title |
Springer eBooks |
776 08 - ADDITIONAL PHYSICAL FORM ENTRY |
Display text |
Printed edition: |
International Standard Book Number |
9783642046087 |
856 40 - ELECTRONIC LOCATION AND ACCESS |
Uniform Resource Identifier |
http://dx.doi.org/10.1007/978-3-642-04609-4 |
942 ## - ADDED ENTRY ELEMENTS (KOHA) |
Source of classification or shelving scheme |
|
Item type |
E-Book |