Quality management and regulation -- Technique and troubleshooting of antibody testing -- Overview of automated immunohistochemistry -- Automated staining - Dako Perspective -- Automated staining - Ventana Perspective -- Tissue microarray -- Unknown primary/undifferentiated neoplasms in surgical and cytologic specimens -- Exfoliative cytopathology -- Predictive markers of breast cancer: ER, PR and Her-2/neu -- Central and peripheral nerve system tumors -- Thyroid and parathyroid gland -- Adrenal gland -- Salivary gland and other head and neck structures -- Lung, pleura, and mediastinum -- Breast -- Uterus -- Ovary -- Prostate gland -- Urinary bladder -- Kidney -- Testis and paratesticular tissues -- Pancreas and ampullae -- Liver, bile ducts and gallbladder -- Upper gastrointestinal tract -- Lower gastrointestinal tract and microsatellite instability (MSI) -- Soft tissue and bone tumors -- Lymph node -- Bone Marrow -- Infectious diseases -- Skin -- Application of Direct Immunofluorescence for skin and mucosal biopsies -- In situ hybridization in surgical and cytologic specimens -- Appendices – Antibody information and protocols (Appendix I using Dako System; Appendix II using Ventana System).

In a conceptually current, quick-reference, Question & Answer format, the Handbook of Practical Immunohistochemistry: Frequently Asked Questions provides standardization of the immunostaining process for each antibody and for each staining panel. With links to the authors Immunohistochemical Laboratory website, this volume creates a current and up-to-date information system on immunohistochemistry. This includes access to tissue microarrays (TMA) of over 5,000 tumors to validate common diagnostic panels and provide the best reproducible data for diagnostic purposes. Chapters are presented in a unique Question and Answer format. One table/IHC panel is provided to address each question. A concise explanatory note follows each table/panel to avoid diagnostic pitfalls. Website links are provided throughout to update the massive information in this field, providing the most current knowledge and the potential for live expert consultation. All chapters are written by nationally/internationally recognized experts in the related area ensuring authority and excellence. Comprehensive yet practical and concise, the Handbook of Practical Immunohistochemistry: Frequently Asked Questions, will be of great value for surgical pathologists, pathology residents and fellows, cytopathologists, and cytotechnologists.